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GLOBAL® BLASTOCYST FAST FREEZE® KIT - EQUIPMED - Tecnología de Vanguardia al Servicio Médico

GLOBAL® BLASTOCYST FAST FREEZE® KIT

Reference:

GFV5-005, 1 kit (5 ml).

For cryopreservation of blastocysts.

Store at 2-8°C and protected from light. One (1) yearfrom the date of manufacture.

Composition: 

1 X 5 ml vial of Fast Freeze® Solution 1 (green cap)

1 X 5 ml vial of Fast Freeze® Solution 2 (blue cap)

2 X 5 ml vials of Fast Freeze® Solution 3 (white cap)

10 X Universal GPS® dishes

Base Components:

Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Glycine, L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic Acid, L-Cystine, L-Glutamic Acid, Glycyl-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, EDTA, Phenol Red, HEPES, Human Serum Albumin* (20 mg/ml), Gentamicin Sulfate* (10 μg/ml), *from therapeutic-grade source material

global® Blastocyst Fast Freeze® Solutions 1, 2, and 3 contain increasing concentrations of glycerol and ethylene glycol together with the base components.

Assay (performed for each Sbatch)

Specification

pH – Fast Freeze® Solution 1

7.1-7.5

pH – Fast Freeze® Solution 2

7.1-7.5

pH – Fast Freeze® Solution 3

7.1-7.5

Osmolality – Fast Freeze® Solution 1

1400-2000 mOsM

Osmolality – Fast Freeze® Solution 2

4900-6600 mOsM

Osmolality – Fast Freeze® Solution 3

7800-10600 mOsM

Endotoxin (LAL) – Fast Freeze® Solution 1

< 1.0 EU/ml

Endotoxin (LAL) – Fast Freeze® Solution 2

< 1.0 EU/ml

Endotoxin (LAL) – Fast Freeze® Solution 3

< 1.0 EU/ml

Sterility Test (bacterial and fungal screen, SAL 10–3)

PASS

Mouse Embryo Assay (% re-expanded blastocysts at 24 h of culture)

> 80%

Universal GPS® Dishes

 

Endotoxin (LAL)

< 20 EU/device

1-cell Mouse Embryo Assay (% expanded blastocysts after 96 h)

> 80%


Cant.