Reference:
GFT5-055 , 1 kit (5 ml).
Rehydration of blastocysts that have been cryopreserved using the global® Blastocyst Fast Freeze® Kit.
Store at 2-8°C and protected from light. One (1) year from the date of manufacture.
Composition:
2 X 5 ml vials of Thaw Solution 1 (white cap)
1 X 5 ml vial of Thaw Solution 2 (blue cap)
1 X 5 ml vial of Thaw Solution 3 (green cap)
1 X 5 ml vial of Thaw Solution 4 (yellow cap)
1 X 5 ml vial of Thaw Solution 5 (red cap)
10 X Universal GPS® dishes
Base Components
Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Glycine, L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic Acid, L-Cystine, L-Glutamic Acid, Glycyl-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, EDTA, Phenol Red, HEPES, Human Serum Albumin* (10 mg/ml), Gentamicin Sulfate* (10 μg/ml), *from therapeutic-grade source material
global® Blastocyst Fast Freeze® Thawing Solutions 1, 2, 3, 4 and 5 contain decreasing concentrations of sucrose together with the base components.
Assay (performed for each batch) |
Specification |
pH – Thawing Solution 1 |
7.1-7.5 |
pH – Thawing Solution 2 |
7.1-7.5 |
pH – Thawing Solution 3 |
7.1-7.5 |
pH – Thawing Solution 4 |
7.1-7.5 |
pH – Thawing Solution 5 |
7.1-7.5 |
Osmolality – Thawing Solution 1 |
1100-1325 mOsM |
Osmolality – Thawing Solution 2 |
650-755 mOsM |
Osmolality – Thawing Solution 3 |
400-525 mOsM |
Osmolality – Thawing Solution 4 |
365-405 mOsM |
Osmolality – Thawing Solution 5 |
270-300 mOsM |
Endotoxin (LAL) –Thawing Solution 1 |
< 1.0 EU/ml |
Endotoxin (LAL) –Thawing Solution 2 |
< 1.0 EU/ml |
Endotoxin (LAL) –Thawing Solution 3 |
< 1.0 EU/ml |
Endotoxin (LAL) –Thawing Solution 4 |
< 1.0 EU/ml |
Endotoxin (LAL) –Thawing Solution 5 |
< 1.0 EU/ml |
Sterility Test (bacterial and fungal screen, SAL 10–3) |
PASS |
Mouse Embryo Assay* (% re-expanded blastocysts at 24 h of culture) |
>80% |
Universal GPS® Dishes |
|
Endotoxin (LAL) |
< 20 EU/device |
1-cell Mouse Embryo Assay (% expanded blastocysts after 96 h) |
> 80% |