GLOBAL® BLASTOCYST FAST FREEZE® THAWING KIT

Reference:

GFT5-055 , 1 kit (5 ml).

Rehydration of blastocysts that have been cryopreserved using the global® Blastocyst Fast Freeze® Kit.

 

Store at 2-8°C and protected from light. One (1) year from the date of manufacture.

 

Composition:

2 X 5 ml vials of Thaw Solution 1 (white cap)

1 X 5 ml vial of Thaw Solution 2 (blue cap)

1 X 5 ml vial of Thaw Solution 3 (green cap)

1 X 5 ml vial of Thaw Solution 4 (yellow cap)

1 X 5 ml vial of Thaw Solution 5 (red cap)

10 X Universal GPS® dishes

 

Base Components

Sodium Chloride, Potassium Chloride, Calcium Chloride, Potassium Phosphate, Magnesium Sulfate, Sodium Bicarbonate, Glucose, Lactate Na Salt, Sodium Pyruvate, Glycine, L-Alanine, L-Arginine HCl, L-Asparagine, L-Aspartic Acid, L-Cystine, L-Glutamic Acid, Glycyl-Glutamine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, EDTA, Phenol Red, HEPES, Human Serum Albumin* (10 mg/ml), Gentamicin Sulfate* (10 μg/ml), *from therapeutic-grade source material

 

global® Blastocyst Fast Freeze® Thawing Solutions 1, 2, 3, 4 and 5 contain decreasing concentrations of sucrose together with the base components.

 

Assay (performed for each batch)	Specification
pH – Thawing Solution 1	7.1-7.5
pH – Thawing Solution 2	7.1-7.5
pH – Thawing Solution 3	7.1-7.5
pH – Thawing Solution 4	7.1-7.5
pH – Thawing Solution 5	7.1-7.5
Osmolality – Thawing Solution 1	1100-1325 mOsM
Osmolality – Thawing Solution 2	650-755 mOsM
Osmolality – Thawing Solution 3	400-525 mOsM
Osmolality – Thawing Solution 4	365-405 mOsM
Osmolality – Thawing Solution 5	270-300 mOsM
Endotoxin (LAL) –Thawing Solution 1	< 1.0 EU/ml
Endotoxin (LAL) –Thawing Solution 2	< 1.0 EU/ml
Endotoxin (LAL) –Thawing Solution 3	< 1.0 EU/ml
Endotoxin (LAL) –Thawing Solution 4	< 1.0 EU/ml
Endotoxin (LAL) –Thawing Solution 5	< 1.0 EU/ml
Sterility Test (bacterial and fungal screen, SAL 10–3)	PASS
Mouse Embryo Assay* (% re-expanded blastocysts at 24 h of culture)	>80%
Universal GPS® Dishes
Endotoxin (LAL)	< 20 EU/device
1-cell Mouse Embryo Assay (% expanded blastocysts after 96 h)	> 80%