GLOBAL® DMSO BLASTOCYST VITRIFICATION KIT

Reference: 

DMV5-005, 1 kit (5 ml).

 

For vitrification (ultra-rapid freezing) and cryostorage of human blastocysts.

 

Store at 2-8°C and protected from light. One (1) year from the date of manufacture.

 

Composition:

1 X 5 ml vial of DMSO Equilibration Solution (blue caps)

1 X 5 ml vials of Vitrification Solution (white caps)

10 X embryo GPS® dishes

 

Base Components:

Sodium Chloride, Potassium Chloride, Calcium Chloride, Sodium Bicarbonate, Glucose, Lactate Na Salt, L-Alanine, L-Arginine HCl, L-Asparagine, L-Glutamic Acid, Glycyl-Glutamine, L-Histidine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Threonine, L-Tryptophan, L-Tyrosine, Phenol Red, HEPES, Gentamicin Sulfate* (10 μg/ml).

 

The solutions in the global® DMSO Blastocyst Vitrification Kit are membrane filtered and aseptically processed according to cGMP procedures which have been validated to meet a sterility assurance level (SAL) of 10-3.
Assay (performed for each batch)	Specification
pH — Equilibration and Vitrification Solutions	7.1-7.5
Osmolality — Equilibration Solution	2600-3500 mOsM
Osmolality — Vitrification solution	4900-6600 mOsM
Endotoxin (LAL) - Equilibration and Vitrification Solutions	< 1.0 EU/ml
Sterility Test (bacterial and fungal screen, SAL 10–3)	PASS
1-cell Mouse Embryo Assay (% expanded blastocysts at 96 h of culture following step-in, step-out of vitrification and warming solutions)	> 80%
embryo GPS® Dishes
Endotoxin (LAL)	< 20 EU/device
1-cell Mouse Embryo Assay (% expanded blastocysts after 96 h)	> 80%