NidOil

In vitro overlay for gametes and zygotes

Some procedures for IVF and ICSI use an oil overlay to protect small volumes of culture medium from evaporation in the incubator and to help dampen the effects of sudden changes in temperature, gas saturation, and pH. Likewise, gamete manipulation under the microscope requires the gametes to be held in small drops of medium under oil, to maintain a constant environment within the culture medium.

Features:

- In vitro overlay for gametes and zygotes.

- Maintain a constant environment within the culture medium.

- Nidacon offers you NidOil, a paraffin oil product designed with gametes and embryos in mind. The oil is neither sticky nor too viscous, to facilitate pipetting, but is sufficiently viscous to prevent movement of the drops of media around the culture dish.

- There have been several reports of paraffin oils becoming embryo-toxic after exposure to light on the laboratory bench. As a precaution against any light-induced changes, NidOil is supplied in amber, screw-top bottles.

Components

– Light paraffin oil.

Performance Characteristics

- Density: 0.82 – 0.88g/mL.

- Endotoxin levels: <0.100 EU/mL.

- Oil, bottles and stoppers are M.E.A. tested.

Cant.